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Third generation Enzyme ImmunoAssay (ELISA) for the determination of antibodies to Human T-cell Lymphotropic Virus type I&II or HTLV I&II Ab. The kit may be used for the screening of blood units and the follow-up of HTLV I&II-infected patients.
Microplates are coated with HTLV I&II-specific peptides (gp46-I, gp46-II and p21-I). The solid phase is first treated with the diluted sample and anti HTLV I&II Ab are captured, if present, by the antigens coated on the microplate.
After washing out all the other components of the sample, in the 2nd incubation bound anti HTLV I&II antibodies, IgG and IgM as well, are detected by the addition of polyclonal specific anti hIgG&M antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti HTLV I&II antibodies present in the sample.
A cut-off value let optical densities be interpreted into anti HTLV I&II antibody negative and positive results.
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HTLV 
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