CTA - IgA

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Enzyme ImmunoAssay (ELISA) for the qualitative determination of  IgA antibodies to Chlamydia Trachomatis in human plasma and sera.  The product is intended for the follow-up of patients showing pathologies referable to Chl. Trachomatis infection. 

Micro-plates are coated with a species-specific polypeptide derived from C.trachomatis major outer membrane antigen.In the 1^st incubation, the solid phase is treated with diluted samples and anti-C.trachomatis IgA are captured, if present, by the antigens.After washing out all the other components of the sample, in the 2^nd incubation bound anti-C.trachomatis IgA are detected by the addition of anti hIgA antibody, labelled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-C.trachomatis IgA antibodies present in the sample. IgA in the sample are then determined by a cut-off value able to discriminate between the negative and the positive population.Interferences due to IgG are blocked by means of a Neutralizing  Reagent directly added to the sample in the well.