CTM - IgM

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Enzyme ImmunoAssay (ELISA) for the determination  of   IgM antibodies to Chlamydia Trachomatisin human plasma and sera.

Micro-plates are coated with a species-specific polypeptide derived from C.trachomatis major outer membrane antigen.In the 1^st incubation, the solid phase is treated with diluted samples and anti-CT IgM are captured, if present, by the antigens.After washing out all the other components of the sample, in the 2^nd incubation bound anti-CT IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CT IgM antibodies present in the sample. The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.Neutralization of IgG anti-CT, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM.