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Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG auto-antibodies to Ro/SSA 60KDa, Ro/SSA 52KDa, La/SSB, RNP-68, Sm, Scl-70, Jo-1, CENP-B, in human plasma and sera.
For in vitro diagnostic use only.
Microplates are coated by strips with ANA recombinant or purified Antigens specific: Ro/SSA52,RoSSA60, La/SSB, RNP-68, Sm, Scl-70, Jo-1, CENP-B.
| Position |
Autoantigen |
Composition |
| A |
SSA60 |
Rec Ag |
| B |
SSA52 |
Rec Ag |
| C |
SSB |
Rec Ag
|
| D |
RNP-68 |
Rec Ag
|
| E |
Sm |
Native Ag |
| F |
Scl-70 |
Rec Ag
|
| G |
Jo-1 |
Rec Ag
|
| H |
CENP-B |
Rec Ag |
In the 1st incubation, the solid phase is treated with diluted samples and anti nuclear IgG are captured, if present, by the solid phase.After washing out all the other components of the sample, in the 2nd incubation bound anti nuclear IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP). The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti nuclear IgG antibodies present in the sample. The presence of IgG in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.
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ANA 8 parameters Profile
