Percorso

HCV IgM

Enzyme ImmunoAssay (ELISA) for the determination of IgM antibodies to Hepatitis C Virus in human plasma and sera.
012eb9976d0786388a196b7d6811c8c9
Description
 

 

Enzyme ImmunoAssay (ELISA) for the determination of IgM antibodies to Hepatitis C Virus in human plasma and sera. The kit is intended for the follow-up of HCV chronic patients submitted to anti-viral pharmaceutical treatment. For "in vitro" diagnostic use only.
Microplates are coated with HCV immunodominant synthetic antigens (core peptide, recombinant NS3, NS4 and NS5 peptides).
In the 1st incubation, the solid phase is treated with diluted samples and anti HCV IgM are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti-HCV IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-HCV IgM antibodies present in the sample.
The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.
Neutralization of IgG anti-HCV, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM.

Download Free Joomla Templates by vonfio.de