Percorso

HEV IgG

Third generation Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG antibodies to Hepatitis E Virus in human plasma and sera
012eb9976d0786388a196b7d6811c8c9
Description

 

 

Third generation Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG antibodies to Hepatitis E Virus in human plasma and sera. The kit may be used for the screening of blood units and the follow-up of HEV-infected patients. For "in vitro" diagnostic use only.
Microplates are coated with HEV-specific synthetic antigens encoding for conservative and immunodominant determinants derived from Mexican and Burma virus strains.
The solid phase is first treated with the diluted sample and anti HEV IgG are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti-HEV IgG are detected by the addition of polyclonal specific anti hIgG antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti HEV IgG present in the sample. A cut-off value let optical densities be interpreted into anti-HEV IgG negative and positive results.

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