Enzyme ImmunoAssay (ELISA) for the determination of IgM antibodies to Chlamydia Trachomatisin human plasma and sera.
For “in vitro” diagnostic use only.

The determination of species-specific IgG, IgM and IgA is a helpful tool for the clinician to identify the infective agent and to decide the right therapy. The kit is intended for the follow up of patients undergoing a Chlamydia trachomatis infection. Microplates are coated with a species-specific polypeptide derived from C.trachomatis major outer membrane antigen.
In the 1st incubation, the solid phase is treated with diluted samples and anti-CT IgM are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti-CT IgM are detected by the addition of anti hIgM antibody, labeled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-CT IgM antibodies present in the sample.
The presence of IgM in the sample may therefore be determined by means of a cut-off value able to discriminate between negative and positive samples.
Neutralization of IgG anti-CT, carried out directly in the well, is performed in the assay in order to block interferences due to this class of antibodies in the determination of IgM