HEV Ab (Version ULTRA) – ELISA

Dia.pro HDV Ab

HEV Ab (Version ULTRA) – ELISA

Third generation Enzyme ImmunoAssay (ELISA) for the qualitative determination of antibodies to Hepatitis E Virus in human plasma and sera. The kit is for use in screening of blood units and the follow-up of HEV-infected patients. For “in vitro” diagnostic use only.
Microplates are coated with HEV-specific synthetic antigens encoding for conservative and immunodominant determinants derived from Mexican and Burma virus strains.
The solid phase is first treated with the diluted sample and HEV Ab are captured, if present, by the antigens.
2nd incubation bound HEV antibodies, IgG and IgM as well, are detected by the addition of polyclonal specific anti hIgG&M antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti HEV antibodies present in the sample. A cut-off value let optical densities be interpreted into HEV antibody negative and positive results.

Dia.pro HDV Ab