Enzyme ImmunoAssay (ELISA) for the quantitative/qualitative determination of IgG antibodies to Herpes Simplex Virus type 1 in human plasma and sera.
For “in vitro” diagnostic use only.
Microplates are coated with native inactivated HSV1. The solid phase is first treated with the diluted sample and IgG to HSV are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti HSV1 IgG are detected by the addition of polyclonal specific anti hIgG antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti HSV1 IgG antibodies present in the sample.
A Calibration Curve, calibrated against an internal Gold Standard, makes possible a quantitative determination of the IgG antibody in the patient.