IgG anti Jo-1 – ELISA

Dia.pro Instrument

IgG anti Jo-1 – ELISA

The device JO1.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against Jo-1 autoantigens in human plasma and sera.

For in vitro diagnostic use only.

Microplates are coated with a preparation of recombinant Jo-1 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti Jo-1 IgG are captured, if present, by the solid phase.After washing out all the other components of the sample, in the 2nd incubation bound anti Jo-1 IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP).

The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti Jo-1 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

Dia.pro Instrument