IgG anti SSA 52 KD – ELISA

Dia.pro Instrument

IgG anti SSA 52 KD – ELISA

The device SSA52.CE is an Enzyme ImmunoAssay (ELISA) intended to be used for the quantitative determination of IgG autoantibodies against SSA-52KD autoantigens in human plasma and sera.

For in vitro diagnostic use only.

Microplates are coated with a preparation of Recombinant SSA 52KDa antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti-SSA52 IgG are captured, if present, by the solid phase.After washing out all the other components of the sample, in the 2nd incubation the bound anti-SSA52 IgG are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti-SSA52 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.

Dia.pro Instrument