IgG anti U1 – sn RNP68 – ELISA
Enzyme ImmunoAssay (ELISA) for the quantitative determination of IgG autoantibodies to RNP-68KDa in human plasma and sera.
For in vitro diagnostic use only.
Microplates are coated with a preparation of recombinant U1-snRNP 68 antigen. In the 1st incubation, the solid phase is treated with diluted samples and anti U1-snRNP 68 IgG are captured, if present, by the solid phase.After washing out all the other components of the sample, in the 2nd incubation bound anti U1-snRNP 68 are detected by the addition of anti hIgG antibody, labeled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti U1-snRNP 68 IgG antibodies present in the sample. IgG in the sample may be quantitated by means of a standard curve calibrated in arbitrary units per milliliter (arbU/ml) as no international standard is available.