Syphilis Ab One Version ULTRA – ELISA

Syphilis Ab One Version ULTRA – ELISA

Enzyme ImmunoAssay (ELISA) for the one-step FAST qualitative determination of antibodies (IgG, IgM and IgA) to Treponema Pallidum  in human plasma and sera.

The kit is intended for the screening of blood units and the follow-up of Tp-infected patients. For “in vitro” diagnostic use only.

Microplates are coated with purified Treponema pallidum synthetic antigens (p15, p17 and p47). Patient’s serum/plasma is added to the microwell together with a mix of Tp synthetic antigens, labelled with peroxidase (HRP).

The specific immunocomplex, formed in the presence of anti Tp Ab in the sample, is captured by the solid phase.
At the end of the one-step incubation, microwells are washed to remove unbound serum proteins and HRP conjugate. The chromogen/substrate is then added and, in the presence of captured immunocomplex, the colorless substrate is hydrolyzed by the bound HRP conjugate to a colored end-product.

After blocking the enzymatic reaction, its optical density is measured by an ELISA reader. The color intensity is proportional to the amount of anti Tp Ab present in the sample. The version ULTRA is suitable for automated screenings