Toxo IgG – ELISA
Enzyme ImmunoAssay (ELISA) for the quantitative/qualitative determination of IgG antibodies to Toxoplasma gondii in plasma and sera.The solid phase is first treated with the diluted sample and IgG to T. gondii are captured, if present, by the antigens.
After washing out all the other components of the sample, in the 2nd incubation bound anti Toxoplasma gondii IgG are detected by the addition of polyclonal specific anti human IgG antibodies, labelled with peroxidase (HRP).
The enzyme captured on the solid phase, acting on the substrate/chromogen mixture, generates an optical signal that is proportional to the amount of anti Toxoplasma gondii IgG antibodies present in the sample. A Calibration Curve, calibrated against the W.H.O 3nd international standard , makes possible a quantitative determination of the IgG antibody in the patient.