Enzyme ImmunoAssay (ELISA) for the qualitative and semi quantitative determination of IgG antibodies to West Nile Virus (WNV) in human plasma and sera.

Diagnosis of WNV is generally obtained through detection of virus-specific IgG and IgM antibodies IgM against the most immunodominant antigens of the virus (ENV and NS1) by means of ELISA techniques. While IgM are a marker of acute infection, IgG develop later on in the course of recovery and convalescence. It is common in serologic testing for cross-reactions to occur among Flaviviruses such as Dengue Virus, Zika Virus and Tick-Borne Encephalitis virus even with the newly released assays based on recombinant antigens; this necessitates caution when evaluating serologic results of Flaviviral infections