Enzyme ImmunoAssay (ELISA) for the qualitative determination of IgG auto-antibodies to SSA, SSB, Sm, RNP68, Scl-70, Jo-1, in human plasma and sera. For in vitro diagnostic use only. Autoimmunity is the failure of an organism to recognize its own constituent parts as self, which allows an immune response against its own cells and tissues. Any disease that results from such an aberrant immune response is termed an Autoimmune Disease. Rheumatoid autoimmune diseases are often associated with auto-antibodies to Nuclear Antigens. We can to distinguish between Anti Nuclear Antibodies (ANA), associate with autoimmune systemic diseases as SLE (Systemic Lupus Erythematosus), RA (Reumatoid Arthritis), Scleroderma, MCDT (Mixed Connective Tissue Disease) and Sjogren's Syndrome; and Extractable anti Nuclear Antibodies (ENA), associate with autoimmune systemic disease as Polymyositis, SLE, MCDT and Sjogren's Syndrome.